Tools of Recombinant DNA Technology
Tools of Recombinant DNA Technology: Overview
This topic describes the tools that are used in recombinant DNA technology. It gives a detailed explanation of restriction enzymes, cloning vectors and competent host with the diagrammatic representations.
Important Questions on Tools of Recombinant DNA Technology
What is stable in plants?
What are the methods of direct gene transfer?
What is gene transfer and its types?
What are the different physical and chemical methods of gene transfer?
Which is an example for direct gene transfer?
Which chemical is used in direct gene transfer?
What is Indirect Method of Gene Transfer?
What is the nomenclature of restriction enzymes?
What is the nomenclature of restriction enzymes?
What is the naming procedure for restriction endonuclease give an example?
What is the nomenclature of restriction enzymes?
Insertion of foreign DNA fragment into the pBR322 using enzyme Pvu I or Pst I place make tetR nonfunctional.
Fill in the blank with the correct answer from the bracket.
The recognition site Pvu I in pBR322 is located within the _____ gene. (tetR, ampR, EcoRI)
Define cloning site.
There are three types of restriction endonuclease enzymes that are used in biotechnology.
Transfer of desired DNA fragments into the host cell is done by _____. (Vector/Liposomes)
Which of the following statement is correct regarding EcoRI restriction endonuclease enzyme?
Molecular scissors or biological scissors refer to
Cloning vectors are called vehicle DNA.
Restriction endonuclease is an enzyme commonly known as genetic glue.
